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RNA Identification of PRIME Cells Predicting Rheumatoid Arthritis Flares

Dana E. Orange ... 其他 • 2020.07.16
• 乌帕替尼和阿巴西普治疗类风湿性关节炎的比较试验 • 吸烟与类风湿性关节炎风险









我们在患者类风湿关节炎发作前1~2周观察到血液转录谱有一致变化。类风湿关节炎患者出现B细胞活化,随后血液内的循环CD45-CD31-PDPN+炎症前间充质细胞(preinflammatory mesenchymal cell,PRIME细胞)扩增;这些细胞具有炎性滑膜成纤维细胞的特征。全部4例患者的循环PRIME细胞水平在发作期间均降低,而流式细胞术和分选细胞RNA-seq在另外19例类风湿关节炎患者证实存在PRIME细胞。





Dana E. Orange, M.D., Vicky Yao, Ph.D., Kirsty Sawicka, Ph.D., John Fak, M.S., Mayu O. Frank, N.P., Ph.D., Salina Parveen, M.A., Nathalie E. Blachere, Ph.D., Caryn Hale, Ph.D., Fan Zhang, Ph.D., Soumya Raychaudhuri, M.D., Ph.D., Olga G. Troyanskaya, Ph.D., and Robert B. Darnell, M.D., Ph.D.
From the Laboratory of Molecular Neuro-oncology, Rockefeller University (D.E.O., K.S., J.F., M.O.F., S.P., N.E.B., C.H., R.B.D.), the Hospital for Special Surgery (D.E.O.), and the Simons Foundation (O.G.T.) — all in New York; Rice University, Houston (V.Y.); Princeton University, Princeton, NJ (V.Y., O.G.T.); Howard Hughes Medical Institute, Chevy Chase, MD (N.E.B., R.B.D.); and the Divisions of Rheumatology and Genetics, Brigham and Women’s Hospital, Harvard Medical School, Boston, and the Broad Institute, Cambridge — both in Massachusetts (F.Z., S.R.). Address reprint requests to Dr. Orange at Rockefeller University, Hospital for Special Surgery, 1230 York Ave., New York, NY 10075, or at dorange@rockefeller.edu, or to Dr. Darnell at Rockefeller University, 1230 York Ave., New York, NY 10075, or at darnelr@rockefeller.edu.



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